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Should dual antibodies (penicillin&streptomycin) be added to the culture medium?

Pubtime：2024-04-23

View volume：212

Dual antibodies are not necessary for cell growth. We do not routinely use antibiotics when cultivating cells, as continuous use of antibiotics can promote the development of antibiotic resistant cell lines, leading to persistent mild contamination. Once antibiotics are removed from the culture medium, this mild contamination will eventually develop into large-scale contamination. The specific situation can be decided whether to use bispecific antibodies based on the environment of one's own laboratory.

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