"Heyuan Liji" is a joint venture between Heyuan Biotechnology (stock code: 688238) and Liji Biotechnology, specializing in the "Liji Biotechnology" and "Life-ilab" reagent brands

Language：

中文 English

He Yuan Li Ji

021-50778506

Hot Search：

Product Center

Publications

Technical Services

Information

Resource Centre

About Li Ji

Back

Cell Biology

Western Blot

Electrophoresis

Fluorescent labeling dye

PCR&q-PCR

Synthesis and extraction of nucleic acids

Microbial culture

Elisa

Biochemical reagents

Instrument consumables

Back

Information

Paper Prize

Back

Services

Vector for gene therapy

Genetic manipulation

Cell experiments

Animal testing

Other special services

Back

Back

Frequently asked questions

Back

About us

Agent

Recruiting wise men

Can pre stained protein markers be stained with Coomassie Brilliant Blue?

Pubtime：2024-08-02

View volume：137

Some pre stained markers can be stained with Coomassie Brilliant Blue, while others cannot. Reason: Coomassie Brilliant Blue contains acidic functional groups, such as two - HSO3 in R-250 molecules. The acidic groups can bind to the basic functional groups of proteins, causing the protein to be colored. The higher the protein content, the darker the color. Pre dyed protein markers are covalently bound to proteins of different sizes and dyes. Depending on the dye, the binding position of the protein is also different. If the dye on the marker binds to the Coomassie Brilliant Blue binding group, it cannot bind to Coomassie Brilliant Blue again for staining. Otherwise, Coomassie Brilliant Blue can bind and stain.

Previous：Why is the pre stained protein marker band also exposed during WB experiments?

Next：What are the precautions for using pre stained protein markers

Group Website