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Can pre stained protein markers be stained with Coomassie Brilliant Blue?

Pubtime：2024-08-02

View volume：179

Some pre stained markers can be stained with Coomassie Brilliant Blue, while others cannot. Reason: Coomassie Brilliant Blue contains acidic functional groups, such as two - HSO3 in R-250 molecules. The acidic groups can bind to the basic functional groups of proteins, causing the protein to be colored. The higher the protein content, the darker the color. Pre dyed protein markers are covalently bound to proteins of different sizes and dyes. Depending on the dye, the binding position of the protein is also different. If the dye on the marker binds to the Coomassie Brilliant Blue binding group, it cannot bind to Coomassie Brilliant Blue again for staining. Otherwise, Coomassie Brilliant Blue can bind and stain.

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