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Why is the pre stained protein marker band also exposed during WB experiments?
Pre stained protein markers are composed of recombinant proteins, and the protein markers used may contain His tags. If the primary antibody uses his tag, the secondary antibody will not only bind to the primary antibody with his tag, but also to the protein marker band with his tag. Therefore, after adding ECL luminescent solution, the protein marker and the target protein in the sample will be exposed together.