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Does the pre dyed protein marker strip fade on the film during the transfer printing process?

Pubtime：2024-08-02

View volume：166

Fading is most likely caused by the detergent in the sealing/washing solution washing some proteins off the membrane. Dyes themselves do not wash off from proteins because they are covalently bound. It has been found that membranes with smaller pore sizes can better retain proteins during closure and washing processes. Therefore, in order to achieve excellent resolution and protein retention, it is recommended to use 0.2 µ m instead of 0.45 µ m membranes. After transfer printing, a pencil can be used to circle the pre dyed strip on the film, so that the position of the strip can still be identified after sealing and processing.

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