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Possible reasons for poor cell death or survival rate of purchased cells?

Pubtime:2024-04-16
View volume:225

Researchers often experience poor survival rates during cell culture, which can be attributed to incorrect use of culture media or poor quality of culture media. Incorrect use of serum or poor quality of serum. Unfreezing process error. After thawing the frozen cells, wash the cells and centrifuge them. Suspended cells are mistaken for dead cells. Incorrect use of cultivation temperature. The cells were placed at -80 ℃ for too long.

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