Frequently Asked Questions - Answers to Reagent Product Issues-He Yuan Li Ji

Q：Quick sealing solution works well, but if left for too long, it will settle and shake again?

It's Tween20. If left at room temperature for a long time, it will slowly oxidize and precipitate. Just shake it. Storing at 4 ℃ will be more stable and prevent precipitation.
Q：Why do flocculent precipitates sometimes appear in serum? Do we need to remove it? How to remove it?

There are various reasons that may lead to the formation of flocculent precipitates, and one of the most common reasons is the aggregation of lipoproteins during the melting process. This phenomenon generally does not affect product quality. Centrifuge at 400 g for 5 minutes, take the supernatant, and then filter. Choose the appropriate pore size of the filter membrane according to the needs, and the most commonly used is the 0.22 um PES material filter membrane. To avoid membrane blockage, it is recommended to centrifuge and add the supernatant to the culture medium for filtration instead of directly filtering the serum.
Q：Which is better in quality between fetal bovine serum and newborn bovine serum?

Fetal bovine serum is of the highest quality because it has not yet been exposed to the outside world, and contains the least harmful components such as antibodies and complement to cells; Bovine serum is the most commonly used natural culture medium in cell culture, containing abundant nutrients necessary for cell growth. It is commonly used for in vitro culture of animal cells and has extremely important functions.
Q：What is the difference between fetal bovine serum and newborn bovine serum?

Fetal cows are sampled from the embryo in the cow's belly, newborn cows are sampled within 0-24 hours of birth, and calves are sampled 10-14 days after birth.
Q：How does Li Ji Biology ensure the quality of premium fetal bovine serum FBS products?

Li Ji Biological FBS undergoes strict screening for each batch, requiring continuous three-layer 0.1um filter membrane filtration. The filter material meets the requirements of the US FDA document, and the entire production process conforms to international fetal bovine serum production. It also undergoes strict quality control and analysis and testing of physical, chemical, and microbiological indicators.
Q：How does the sensitivity of the four mycoplasma detection kits from Yuan Li Ji compare?

A4：1、 The detection sensitivity of the "probe method" is high, and its detection lower limit can reach 5.6-10.8 copies/2 μ L; 2. Next is the "PCR method", which has a higher detection sensitivity similar to or slightly lower than the "probe method"; 3. The detection sensitivity of the "rapid method" is lower than that of the "PCR method" and the "probe method", with a detection lower limit of approximately 22.5-172.3 copies/2 μ L; 4. The detection sensitivity of the "luminescence method" is the lowest, with a detection lower limit of approximately 2200 live bacteria/μ L at a dilution of 10 ^ (-3).
Q：How does He Yuan Li Ji ensure the quality of fetal bovine serum products?

A3: Every batch of H&Y Li Ji FBS is strictly screened, requiring continuous 3-layer 0.1um filter membrane filtration. The filter material meets the requirements of the US FDA document, and the entire production process complies with international fetal bovine serum production. It also undergoes strict quality control and analysis and testing of physical, chemical, and microbiological indicators.
Q：What are the differences between the three transfection reagents used by Yuan Liji and Yuan Liji? How does it compare to Li * *?

A2: Our company conducted self testing and collected customer feedback data, and came to a similar conclusion: in terms of efficiency: EZ Trans Plus>Li * * 2000 ≈ EZ Trans (high efficiency)>Li * * 3000>EZ Trans (low toxicity). Toxicity: EZ Trans (low toxicity) ≈ Li * * 3000<EZ Trans Plus<EZ Trans (high efficiency) ≈ Li * * 2000.
Q：There are 3 types of ECL luminescent liquid, how to choose?

A1: There are three types of luminescent liquids with different sensitivities from Yuan Liji. The enhanced, ultra sensitive, and Fick level correspond to low Pic level, Pic level, and Fick level protein detection, respectively. They can completely replace PE, Millipore, or Thermo's corresponding products. Customers can choose according to the experimental situation, and Liji's best-selling type is the ultra sensitive type.

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