Product Description
The CCK-8 Cell Proliferation and Toxicity Detection Kit is a fast and highly sensitive colorimetric detection kit based on WST-8 (chemical name: 2- (2-methoxy-4-nitrophenyl) -3- (4-nitrophenyl) -5- (2,4-disulfobenzene) -2H-tetrazole monosodium salt), widely used for cell proliferation and cytotoxicity. The detection principle is that WST-8 can be reduced by some dehydrogenases in mitochondria in the presence of electron coupling agent 1-Methoxy PMS to generate orange yellow water-soluble formazan (see Figure 1). The color intensity of the generated formazan is directly proportional to cell proliferation and inversely proportional to cytotoxicity. Use an enzyme-linked immunosorbent assay (ELISA) to measure the OD value at a wavelength of 450nm, indirectly reflecting the number of live cells.
This reagent kit can be used for cell proliferation detection, cytotoxicity detection, drug screening, and cell growth inhibition detection.
图1：WST-8分子结构及检测原理
ordering information
Composition | Article number | Specifications |
CCK-8 reagent kit（Cell Counting Kit-8） | AC11L054 | 500T |
CCK-8 reagent kit（Cell Counting Kit-8） | AC11L054 | 2*500T |
CCK-8 reagent kit（Cell Counting Kit-8） | AC11L054 | 20*500T |
Product advantages
1、Safe and low toxicity:After use, there is no damage to cells, no change in cell morphology, and continuous observation is possible;
2、Good stability:Li Ji Biological CCK-8 is packaged in white bottles and has stable quality;
3、High sensitivity:Compared to MTT method, the sensitivity is increased by 1000 times and the repeatability is good;
4、Easy to operate:Ready to use, with just one step to detect results;
5、Quick response:It can be detected in half an hour;
6、economical and practical:20 * 500T only costs 1500 yuan.
7、The case list can be found on the official website - Resource Center - Case Appreciation Support Query!
Transportation and Preservation
Blue ice transportation. Short term storage at 4 ℃, valid for 3 months- Long term storage at 20 ℃, valid for 24 months. [Note]: Repeated freeze-thaw cycles can cause an increase in the measured background OD value.
This product is limited to scientific experimental research and cannot be used in clinical diagnosis, treatment, or other fields.
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