Product Description
The molecular weight standard for tricolor pre stained proteins includes 11 highly purified and pre stained recombinant proteins (10, 15, 20, 25, 30, 40, 50, 72100130250KD) from 10KD to 250KD. Among them, 72KD bands are orange, 25KD bands are green, and the remaining bands are blue, making them suitable as protein molecular weight standards for SDS-PAGE and Western. This color pre stained protein molecular weight standard has been prepared in 1 x SDS-PAGE loading buffer and can be used directly without boiling, dilution, or the need for reducing agent treatment. According to the size of the sample well, the molecular weight standard for color pre stained proteins is usually 5-10 μ L per sample (5 μ L of 5 × 1.5 mm gel well is sufficient), and very clear protein bands can be observed during electrophoresis, after electrophoresis, and after membrane transfer.
ordering information
Product Name | Article number | Specifications |
Tri color pre stained protein Marker (10-250KD) | AP13L062 | 250μL |
Tri color pre stained protein Marker (10-250KD) | AP13L063 | 5*250μL |
Transportation and Preservation
Blue ice transportation. Short term storage at 4 ℃, valid for 2 months- Long term storage at 20 ℃, valid for 36 months.
usage method
1. After thawing at room temperature, gently mix or use a pipette to slowly blow until evenly mixed, without boiling.
2. Take 5 μ L of this product and test sample for polyacrylamide gel electrophoresis at the same time. It is recommended that qualified laboratories use this product for the first time
Based on one's own experimental conditions and habits, determining the appropriate sample size through pre experiments can save costs and improve experimental images.
matters needing attention
1. This product is limited to scientific experimental research and cannot be used in clinical diagnosis, treatment, or other fields.
2. The inner pore size of Page gel varies with the degree of gel. At low concentrations of gel, the standard migration rate of small molecular weight proteins is greater than that of the indicator, and they will swim at the leading edge of the dye,
The standard spacing for size and quality is also relatively small.
3. When doing Western blot, high molecular weight proteins require longer membrane transfer time or higher membrane transfer pressure, and the protein standard is also the same.
4. Customers who purchase freeze-dried Marker products and need to prepare their own cryopreservation solution can refer to the formula: 67 mM Tris H3PO4, pH 7.5, 5mM EDTA,
2 % (W/V) SDS, 33 % (V/V) Glycerol, 0.02 % (V/V) proclin300。
5. Pre dyed proteins may exhibit different buffering effects due to the binding of dyes to proteins, resulting in varying charges carried by dye molecules in different buffering systems
Under the impact system, there are different apparent molecular weights, and different positions can refer to the official website image。
6. For WB FAQ analysis and solutions, see the series of Protein Electrophoresis on Heyuan Liji WeChat official account.
This product is limited to scientific experimental research and cannot be used in clinical diagnosis, treatment, or other fields.
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