"Heyuan Liji" is a joint venture between Heyuan Biotechnology (stock code: 688238) and Liji Biotechnology, specializing in the "Liji Biotechnology" and "Life-ilab" reagent brands
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The EZ Trans RNA transfection reagent (hereinafter referred to as EZ Trans) uses a novel liposome nanomaterial and can be used to transfect small molecule RNA and DNA within 150bp, such as siRNA and miRNA. The transfected cells include the vast majority of adherent cells, such as general cell lines and tumor cell lines.
This transfection reagent is a fast, efficient, and ready to use solution. Simply mix the cell culture medium with siRNA or miRNA and the transfection reagent in an EP tube, incubate at room temperature for about 2 minutes, and then add it to the complete cell culture medium. Serum has no effect on transfection efficiency, and there is no need to deliberately add or replace the culture medium during the experimental process.
Product Name | Specifications |
EZ Trans RNAtransfection reagent | 1 mL |
Blue ice transportation. Stored at 4 ℃, with a shelf life of 12 months. [Note]: Cannot be frozen!
This product is only for scientific experimental research and should not be used in clinical diagnosis, treatment, or other fields.
A:能不加尽量不加,理论上抗生素进不到细胞内,但是在转染试剂的作用下,细胞的通透性增加,抗生素可能进到细胞内,对实验结果完成影响。
Q:protocol提到,要用Opti-MEM稀释质粒和转染试剂。不能用1640稀释么?另外质粒和转染试剂为什么要先稀释然后再混合?不能把转染试剂直接加到质粒溶液中混合,然后再加培养基稀释么?
Q:protocol提到,“24-48小时内检测转染效率”指的是去除含复合物的培养液换成含血清的培养基之后的24-48h,还是指加入复合物后转染24-48h?
A:所有时间都是从EZ Trans-DNA复合物与细胞接触开始算。
Q:转染效率低:制备DNA-转染试剂复合物时存在干扰或抑制因素
A:由于血清和抗生素会干扰DNA-转染试剂复合物的形成,在制备时,请使用无抗生素、无血清培养基或Opti-MEM培养基
A:在DNA转染和DNA-siRNA共转染过程中,细胞密度控制在60%-70%之间;在siRNA转染过程中,细胞密度控制在50%左右。
A:建议每次转染时,设定一个阳性对照,如绿色荧光蛋白(GFP)。
A:EZ Trans细胞转染试剂应保存在4℃。转染复合物的最佳放置试剂和温度是室温条件下10-20分钟,长时间放置会显著地降低转染效率,请务必控制放置时间在20分钟之内。
A:在形成转染复合物时,标准操作步骤推荐数秒内涡旋混合转染试剂和DNA或siRNA以生产高效转染复合物。如果想将粘附于管壁的复合物离心,请务必使用极低转速离心,如80g离心5秒。
A:EZ Trans细胞转染试剂的转染效率不受培养液中的血清影响,因此建议转染过程中细胞的培养液使用含有10%胎牛血清和抗生素的完全培养液以改善细胞的状态。
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