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EZ 555 TUNEL Cell Apoptosis Detection Kit (Orange Red Fluorescence)

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Product Item Number

Product Specifications

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AC12L056

20T

1180
AC12L057

50T

2280

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Product Description

A significant characteristic of cell apoptosis is the degradation of chromosomal DNA, which is a common phenomenon. This degradation is very specific and regular, and the resulting DNA fragments of different lengths are about 180 bp-200 bp integer multiples, which is shown by the unique ladder pattern in the agarose gel electrophoresis. This kit adopts the TUNEL method, and uses Terminal Deoxyribonucleotide Transfer (TdT) to catalyze the incorporation of EZ 555 dUTP into the 3 ´ - OH end of apoptotic cell broken DNA. The DNA labeled with EZ 555-dUTP can be directly observed under a fluorescence microscope or quantified using a flow cytometer. The TUNEL method can selectively detect apoptotic cells, rather than necrotic cells or cells with DNA strand breaks caused by irradiation and drug therapy. In the TUNEL experiment, TdT enzyme catalyzes the incorporation of dUTP into the 3 ′ - OH end of a broken DNA strand. Antigen labeled dUTP (such as digoxin dUTP, biotin dUTP) is a faster and more direct detection method because it can be directly detected in situ.

Product components

Components	20 T	50 T
A.EZ 555 TUNEL Reaction Buffer	1 mL	2 × 1.25 mL
B.TdT Enzyme	20 μL	50 μL
C.Proteinase K (2 mg/mL)	40 μL	100 μL
D.DNase I (2 U/μL)	5 μL	13 μL
E.10 × DNase I Buffer	100 μL	260 μL

Transportation and storage

Blue ice transportation- Store at 20 ℃ away from light, with a shelf life of 24 months. [Note]: Avoid repeated freezing and thawing.

This product is only for scientific experimental research and should not be used in clinical diagnosis, treatment, or other fields.

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