Product Description
Lysosomes are organelles with a single-layer membrane sac like structure inside cells. Lysosomes are metaphorically referred to as the "enzyme warehouse" or "digestive system" inside cells. Lysosomes contain many types of hydrolytic enzymes that can digest large particles such as food or pathogenic bacteria ingested by cells into biomolecules. The residues are excreted from cells through efflux, decomposing many substances. They can also digest certain aging organelles and biomolecules trapped in lysosomes during cell differentiation. The pH value inside the lysosome membrane is about 4.5-4.8, allowing digestive enzymes to work under acidic conditions. In the cytoplasm outside the lysosome, the pH value is around 7.2, and the lysosome membrane maintains this pH difference inside and outside the lysosome by pumping H+from the cytoplasm.
The Smart Cell Lysosome Staining Kit * Orange * can label lysosomes in live cells with orange fluorescence (Ex/Im=542/556 nm). This reagent kit uses a hydrophobic complex dye, which belongs to lysosomal dyes. The dye penetrates into intact living cells, selectively enters lysosomes, and is locked in lysosomes due to different pH environments inside and outside the lysosome membrane. Once the dye enters the lysosome, the fluorescence is significantly enhanced and very stable. Based on this characteristic, this kit can be used for applications such as enzyme-linked immunosorbent assay, immunohistochemistry, and flow cytometry, and can be applied in various types of studies, including cell adhesion, chemotaxis, multidrug resistance, cell activity, apoptosis, and cytotoxicity. It is suitable for both proliferating and non proliferating cells, as well as for suspended and adherent cells.
The Smart Cell Lysosome Staining Kit is a type of fluorescence imaging tool specially developed by Life iLab company. The kit is designed rigorously and requires very few manual steps. Can be used to label organelles, subcellular structures, such as cell membranes, lysosomes, lysosomes, and nuclei. The Smart Cell Lysosome Staining Kit provides a complete set of reagents for labeling lysosomes, offering different fluorescence options such as blue/green/red/orange for each cell structure. It can be used alone or supports multiple fluorescence analysis of cells. This efficient cell labeling provides a highly effective method for studying cellular activities that occur both spatially and temporally.
Product components
Components | quantity |
Component A: LysoBrite Orangedyestuff | 100 μL (500 X DMSO stock solution) |
Component B: Living cell staining buffer | 50 mL |
Transportation and storage
Blue ice transportation- Store at 20 ℃ away from light and moisture, with a shelf life of 12 months.
This product is only for scientific experimental research and should not be used in clinical diagnosis, treatment, or other fields.
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