Product Description
293 [HEK-293] (Chinese name: human embryonic kidney cells) is derived from the epithelial cells of human embryonic kidneys. The normal cell morphology is epithelioid and grows adherent to the wall.
ordering information
Product Name | Item number | Specifications |
Hek293 | AX01L431 | T25/1 mL |
type of shipping
Transport at room temperature/dry ice.
usage method
Cultivation methods
culture medium | DMEM （GIBCO: C11965500BT）90%, Fetal Bovine Serum (GIBCO: 10099-141) or Foetal Bovine Serum (SKU: AC03L055) 10% |
Cultivation conditions | 37℃ 5% CO2 |
Digestive conditions | 0.25% (w/v) Trypsin-0.53 mM EDTASolution, digestion1-3min |
Passage ratio | 1：5-1：10 |
Fluid change time | Change the fluid 2-3 times a week |
Freezing method
Frozen storage solution | 50% medium, 40% FBS, 5% (v/v) DMSO (AMRESCO: N182) or Heyuan Liji rapid cell cryopreservation solution (serum-free, protein free) (item number: AC05L033) |
Freezing density | 1-3 ×106 /Tube, stored in liquid nitrogen |
For specific operation methods, please refer to the cell user manual
matters needing attention
This product is only for scientific experimental research and should not be used in clinical diagnosis, treatment, or other fields.
After receiving the cells, please carefully read the cell instructions, operate according to the regulations, and record the cell status (take photos). Our company provides customers with a one week after-sales guarantee, and in certain circumstances, cells can be resent for free. If you encounter technical problems, please contact 021-50778506 for technical support and provide pictures life-ilab@obiosh.com .
Customization is available as needed. If you have further modifications (such as gene silencing, gene knockout/knock in, gene overexpression, etc.) to the purchased cell line, please contact Heyuan Biotechnology Service at 021-585887-805615800353038.
reference
Xie QW, et al. Complementation analysis of mutants of nitric oxide synthase reveals that the active site requires two hemes. Proc. Natl. Acad. Sci. USA 93: 4891-4896, 1996. PubMed: 8643499
Da Costa LT, et al. Converting cancer genes into killer genes. Proc. Natl. Acad. Sci. USA 93: 4192-4196, 1996. PubMed: 8633039
Graham FL, et al. Characteristics of a human cell line transformed by DNA from human adenovirus type 5. J. Gen. Virol. 36: 59-72, 1977. PubMed: 886304
Graham FL, et al. Defective transforming capacity of adenovirus type 5 host-range mutants. Virology 86: 10-21, 1978. PubMed: 664220
Harrison T, et al. Host-range mutants of adenovirus type 5 defective for growth in HeLa cells. Virology 77: 319-329, 1977. PubMed: 841862
This product is only for scientific experimental research and should not be used in clinical diagnosis, treatment, or other fields.
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