Product Description
HCT-15 (Chinese name: Human Colorectal Adenocarcinoma Cell), derived from colorectal adenocarcinoma, has a normal epithelial like cell morphology and grows adherent to the wall.
ordering information
Product Name | Item number | Specifications |
HCT-15 | AX04L241 | T25/1 mL |
type of shipping
Transport at room temperature/dry ice.
usage method
Cultivation methods
culture medium | RPMI-1640(Gibco: C11875) 90%, Fetal Bovine Serum (high-quality fetal bovine serum) or Foetal Bovine Serum (item number: AC03L055) 10% |
Cultivation conditions | 37℃ 5% CO2 |
Digestive conditions | 0.25% (w/v) Trypsin-0.53 mM EDTA solution, digest for 1-3 minutes |
Passage ratio | 1：2-1：3 |
Fluid change time | Change the fluid every 2-3 days |
Freezing method
Frozen storage solution | 95% complete culture medium, 5% (v/v) DMSO or Heyuan Liji rapid cell cryopreservation solution (serum-free, protein free) (item number: AC05L033) |
Freezing density | 1-3 ×106 /Tube, stored in liquid nitrogen |
For specific operation methods, please refer to the cell user manual
matters needing attention
This product is only for scientific experimental research and should not be used in clinical diagnosis, treatment, or other fields.
After receiving the cells, please carefully read the cell instructions, operate according to the regulations, and record the cell status (take photos). Our company provides customers with a one week after-sales guarantee, and in certain circumstances, cells can be resent for free. If you encounter technical problems, please contact 021-50778506 for technical support and provide pictures life-ilab@obiosh.com .
Customization is available as needed. If you have further modifications (such as gene silencing, gene knockout/knock in, gene overexpression, etc.) to the purchased cell line, please contact Heyuan Biotechnology Service at 021-585887-805615800353038.
reference
Chen TR, et al. DLD-1 and HCT-15 cell lines derived separately from colorectal carcinomas have totally different chromosome changes but the same genetic origin. Cancer Genet. Cytogenet. 81: 103-108, 1995. PubMed: 7621404
Trainer DL, et al. Biological characterization and oncogene expression in human colorectal carcinoma cell lines. Int. J. Cancer 41: 287-296, 1988. PubMed: 3338874
Dexter DL, et al. N,N-dimethylformamide-induced alteration of cell culture characteristics and loss of tumorigenicity in cultured human colon carcinoma cells. Cancer Res. 39: 1020-1025, 1979. PubMed: 427742
Bender CM, et al. Inhibition of DNA methylation by 5-Aza-2′-deoxycytidine suppresses the growth of human tumor cell lines. Cancer Res. 58: 95-101, 1998. PubMed: 9426064
Vermeulen SJ, et al. Did the four human cancer cell lines DLD-1, HCT-15, HCT-8, and HRT-18 originate from one and the same patient?. Cancer Genet. Cytogenet. 107: 76-79, 1998. PubMed: 9809040
Related product recommendations
Foetal Bovine Serum (SKU: AC03L055)
RPMI-1640 medium (containing L-glutamine) (item number: AC01L064)
This product is only for scientific experimental research and should not be used in clinical diagnosis, treatment, or other fields.
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