Product Description
EZ ECL chemiluminescence solution (regular type) is a conventional sensitivity chemiluminescence (ECL) HRP substrate that can help users achieve 10pg protein detection (~1 × 10-11g) during immunoblotting analysis, greatly saving antibodies. The EZ ECL substrate can provide bright signals and Pick level detection sensitivity for immunoblotting experiments using horseradish peroxidase (HRP) conjugates. This substrate is compatible with various membranes, blocking solutions, and wide range antibody dilutions, meeting users' immunoblotting application needs with excellent performance, versatility, and high cost-effectiveness.
Characteristics of EZ ECL substrate:
Conventional sensitivity: detecting 10pg protein bands on nitrocellulose or PVDF membranes..
Long signal duration: Under optimized conditions, the imprinted bands incubated with substrates can continuously output detectable light signals for 6-8 hours.
Reagent stability: The reagent kit can be stored stably at room temperature for up to 6 months, and at 4 ℃ for 1 year without affecting its use.
Suggested dilution concentration:
Primary antibody concentration: It is recommended to start with 1mg/mL stock solution at a ratio of 1:1000-1:5000 or 0.2-1ug/mL
Secondary antibody concentration: It is recommended to start with 1mg/mL stock solution at a ratio of 1:5000-1:20000 or 2.5-10Ng
ordering information
Product Name | Item number | Specifications |
EZ ECL chemiluminescence solution (ordinary type) | AP34L044 | 100 mL |
EZ ECL chemiluminescence solution (ordinary type) | AP34L045 | 500 mL |
Product components
Components | AP34L044 | AP34L045 |
Ordinary type A liquid | 50 mL | 250 mL |
Ordinary type B liquid | 50 mL | 250 mL |
type of shipping
Blue ice transportation. Store at 4 ℃, away from light and dry, with a shelf life of 12 months.
This product is only for scientific experimental research and should not be used in clinical diagnosis, treatment, or other fields.
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