"Heyuan Liji" is a joint venture between Heyuan Biotechnology (stock code: 688238) and Liji Biotechnology, specializing in the "Liji Biotechnology" and "Life-ilab" reagent brands
click to copy
The Coomassie Brilliant Blue Protein Gel Rapid Staining Solution (Protein Rapid Staining Solution) developed and produced by our company is a type of dye that, like traditional Coomassie Brilliant Blue Staining Solution, quickly binds positively charged groups on proteins, allowing for instantaneous staining of proteins at room temperature.
1. Easy to operate, 2-5 minutes staining, no need for decolorization; Especially for protein purification experiments, the next step of the experiment can be determined within a few minutes based on the results of protein staining bands;
2. It is easy to preserve after preparation, and the decolorized gel can be preserved in pure water for several weeks;
3. Green and environmentally friendly, non-toxic and odorless;
4. Widely applicable, suitable for laboratory prepared or pre made SDS-PAGE and Native gels, and can also be used for staining residual proteins on Western transfer PAGE gels.
Product Name | Specifications |
Coomassie Brilliant Blue Protein Gel Rapid Staining Solution | 500 mL |
Transport at room temperature. Stored at room temperature, with a shelf life of 12 months.
This product is only for scientific experimental research and should not be used in clinical diagnosis, treatment, or other fields.
Q:How is the safety of the product?
A:Traditional dye solutions contain toxic and irritating methanol. We use non-toxic ethanol as a substitute, which has no irritating odor and is safer.
Q:What are the advantages of this dye solution when conducting mass spectrometry or protein purification experiments?
A:If mass spectrometry is performed, it is necessary to pursue weak band staining. Traditional staining has certain advantages, but the traditional staining operation is complex and unsafe to use; If you are conducting protein purification experiments and want to quickly see the purification results, our dye solution is the most suitable product.
Q:After dyeing, the background of the glue is heavy and will turn blue. What should I do? Can it be washed with decolorization solution?
A:The background turns blue, which is a normal phenomenon. Simply place the glue in clean water and purify the background. If using a decolorizing solution for washing, the protein dye will also be washed off.
Q:What should I do if the weak band staining is shallow?
A:This fast dye solution usually takes 2 minutes to develop color, but if it is a weak band and the dyeing time is not enough, the color will be very light, and fading may occur when water is added too early. It is generally recommended to dye overnight, change the water the next day, and purify the background.
Q:Is there a relationship between dyeing effect and adhesive thickness?
A:In general, the thinner the adhesive, the faster and better the dyeing process. If your glue is thick, it is recommended to extend the dyeing time.
Q:Is it normal for trace precipitation to appear in the dye solution? How should we respond?
A:The occurrence of trace precipitation is normal and does not affect the effectiveness of use. During production, in order to enhance the specificity of the dye solution, only the protein is dyed and not the gel. One component is intentionally saturated, but the saturated solution will slightly change with temperature, resulting in a change in solubility and possible trace precipitation. However, as the temperature rises, it will dissolve without affecting its use.
Q:How many times can the dye be reused at most?
A:In principle, the dye solution can be repeatedly used, but if it is used more than 3 times, the amount of SDS remaining in the dye solution may increase, resulting in silver flocculent or trace precipitation. In this case, filtering once is sufficient and does not affect its use. For the best experimental results, we recommend using it up to 3 times repeatedly. It is recommended to extend the staining time for the second and third staining sessions.
Fill in the product batch number for querying
Fill in the product batch number for querying
Hot-sale Product
Hot Articles