Product Description
This product utilizes the principle that the osmotic pressure difference between inside and outside the cell can cause the cell membrane to rupture to lyse red blood cells. This product is mainly used for the separation and purification of tissue cells dispersed by enzymatic digestion, the separation and purification of lymphocytes, and the removal of red blood cells in experiments such as tissue cell protein and nucleic acid extraction.
ordering information
Product Name | Item number | Specifications |
Red blood cell lysate | AP01L106 | 100 mL |
Transportation and storage
Transport at room temperature. Stored at 4 ℃, with a shelf life of 12 months.
usage method
1. Lysis of red blood cells
(1) Add 3 times the volume of red blood cell lysis buffer (such as 1mL of fresh whole blood mixed with 3mL of red blood cell lysis buffer) to 1 part of fresh whole blood to be lysed, gently vortex or invert and mix well; [Note]: If fresh tissue cells are to be processed, they need to be digested with trypsin or other enzymes to form a single cell suspension, centrifuged to collect the cells, and then subjected to subsequent operations.
(2) Incubate on ice for 15 minutes, gently vortex and mix twice during this time; [Note]: After red blood cell lysis, the solution should be clear and transparent.
2. Collect white blood cells
(1) Collect white blood cells by centrifugation at 450 × g for 10 minutes at 4 ℃, and carefully discard the supernatant;
(2) Add twice the volume of red blood cell lysate to the above precipitate, gently vortex and resuspend white blood cells thoroughly (if the initial blood volume is 1 mL, add 2 mL of red blood cell lysate);
(3) Collect white blood cells by centrifugation at 450 × g for 10 minutes at 4 ℃, and carefully and thoroughly aspirate the supernatant;
(4) Resuspend cells for subsequent experiments. [Note]: If extracting RNA, it is best to start using a solution without RNase from this step.
matters needing attention
1. This product is only for scientific experimental research and should not be used in clinical diagnosis, treatment, or other fields.
2. For your own safety, please take protective measures such as wearing lab coats and gloves before using the reagents.
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This product is only for scientific experimental research and should not be used in clinical diagnosis, treatment, or other fields.
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