"Heyuan Liji" is a joint venture between Heyuan Biotechnology (stock code: 688238) and Liji Biotechnology, specializing in the "Liji Biotechnology" and "Life-ilab" reagent brands
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After fixing cells or tissues with polyformaldehyde, formaldehyde, or other aldehyde reagents, it can lead to the formation of aldehyde bonds and carboxymethyl bonds within the cells, which partially block the antigenic determinants. At the same time, cross-linking occurs between proteins, making the antigenic determinants hidden, resulting in weakened staining signals during immunostaining, and even some false negative staining results. Therefore, when performing immunohistochemical staining, antigen repair or exposure is required first, that is, the cross-linking formed between molecules during fixation is destroyed, and the original spatial morphology of the antigen is restored. Thereby improving the detection rate of antigens, reducing background staining, and enhancing the accuracy of detection.
Citrate Antigen Retrieval Solution (50x) is a commonly used antigen retrieval solution that can be used for antigen retrieval of paraffin sections, frozen sections, and other samples fixed with polyformaldehyde, formaldehyde, or other aldehyde reagents. It can effectively remove the cross-linking between proteins caused by aldehyde fixation reagents, fully expose the antigen epitopes in paraffin sections and other samples, and greatly improve the immunostaining effect.
Normally, paraffin sections require antigen repair treatment, while frozen sections may not require antigen repair treatment. Antigen repair can improve the immunostaining effect of paraffin sections and also enhance the staining effect of frozen sections to varying degrees. When the immunostaining effect of frozen sections is not ideal, consider antigen repair.
According to the requirement of 10ml antigen repair solution (1 x) for each slide, 100ml antigen repair solution (50 x) can be used for antigen repair of 500 samples.
Product Name | Specifications |
Sodium Citrate Repair Solution/Sodium Citrate Repair Buffer (50X) | 100mL |
Transport at room temperature. Sealed storage at room temperature, with a shelf life of 12 months.
This product is only for scientific experimental research and should not be used in clinical diagnosis, treatment, or other fields.
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