Product Description
Anti fluorescence quenching sealing agent is a kind of agent that can slow down fluorescence quenching and solidify the glass slide to form a permanent hard seal. The cured glass slide does not need to use nail polish or other sealants to seal the edge of the cover glass slide; The anti fluorescence quenching sealing agent containing DAPI can directly perform nuclear staining.
ordering information
Product Name | Item number | Specifications |
Anti fluorescence quenching sealing agent | AC28L532 | 5 mL |
Transportation and storage
Transport at room temperature. Store at 4 ℃ away from light, with a shelf life of 36 months.
usage method
1. Cell samples
(1) After dyeing, remove the liquid.
(2) Drip 10-20 μ L of sealing agent onto each slide, cover with a cover slip with cells attached, and allow the cells to come into contact with the sealing agent. When covering the cover slip, squeeze out excess sealing agent and try not to have bubbles.
(3) Fluorescence microscopy observation.
(4) After observation, place at 4 ℃ or room temperature and seal overnight, avoiding light.
2. Tissue sectioning
(1) After staining is completed, remove the staining solution.
(2) Drip 10-20 μ L of sealing agent onto the tissue slice, cover it with a cover glass, and allow the slice to fully contact the sealing agent. When covering the cover glass, excess sealing agent can be squeezed out to avoid the formation of bubbles as much as possible.
(3) Observe tissue sections under a fluorescence microscope.
(4) After observation, place at 4 ℃ or room temperature and seal overnight, avoiding light.
3. Other samples
Other samples can be operated according to the above samples.
matters needing attention
1. This product is only for scientific experimental research and should not be used in clinical diagnosis, treatment, or other fields.
2. Before use, restore it to room temperature, gently shake and mix well to avoid the formation of bubbles.
3. This product has poor anti quenching effect on staining live cell membrane dyes and mitochondrial dyes, and is recommended for fixing permeabilized cells or tissues.
4. It may not be applicable to certain dyes, and it is recommended to conduct pre experiment testing for compatibility before the experiment.
5. During the sealing process, avoid areas with excessive moisture, as the sealing agent may inhale too much water vapor, which can affect the sealing effect.
6. For your safety and health, please wear lab clothes and disposable gloves when operating.
Related product recommendations
Universal tissue cell fixative (4% paraformaldehyde) (item number: AC28L112)
Sodium Citrate Repair Solution/Sodium Citrate Repair Buffer (50X) (Product Code: AC28L123)
This product is only for scientific experimental research and should not be used in clinical diagnosis, treatment, or other fields.
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