Product Description
TAE is a widely used nucleic acid electrophoresis buffer in biology, which is mainly used for DNA agarose gel electrophoresis. When using TAE as the electrophoresis buffer, the migration rate of double stranded linear DNA is faster during electrophoresis. When the fragment is larger than 13 kb, it is generally recommended to use TAE for electrophoretic separation. TAE is suitable for the recovery of DNA fragments, but due to its small buffer capacity, it is not recommended for long-term electrophoresis (such as overnight). When the resolution requirement is not high, both TAE and TBE can be used; When the resolution requirement is high, a lower concentration of gel is beneficial for improving the resolution of high molecular weight nucleic acids, and TAE should be used at this time; A higher concentration of gel is beneficial for improving the resolution of small molecular weight nucleic acids, and TBE should be used at this time.
The main components of TAE buffer are Tris acetate and EDTA-2Na. The concentration of Tris acetate and EDTA-2Na in 1 × TAE buffer is 40 mM and 1 mM, respectively.
This product is a white powder, and each bag of TAE powder can be prepared with 1L 1 × TAE buffer solution, which is very convenient to operate.
ordering information
Product Name | Item number | Specifications |
1 x TAE buffer solution (powder) | AN34L157 | 10*1 L |
Transportation and storage
Transport at room temperature. Stored at room temperature, with a shelf life of 24 months.
usage method
1. Take about 600 mL of distilled water and add it to a beaker, then place a magnetic stirrer in the beaker.
2. Place the beaker on a magnetic stirrer and slowly add the entire contents of 1 bag of TAE powder, stirring the solution until completely dissolved.
3. Add distilled water to the TAE solution in step 2 and dilute to 1 L, which is 1 ×.
matters needing attention
1. This product is only for scientific experimental research and should not be used in clinical diagnosis, treatment, or other fields. It should not be used in food or medicine, and should not be stored in ordinary residential areas.
2. For your safety and health, please wear personal protective equipment and clothing when operating.
Related product recommendations
Tris MOPS-SDS electrophoresis buffer (powder) (item number: AP16L545)
1 x TBE buffer solution (powder) (item number: AN34L167)
This product is only for scientific experimental research and should not be used in clinical diagnosis, treatment, or other fields.
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