Product Description
Cyanine 5 has a wide range of applications, not only for labeling biomolecules, but also as a derivatization reagent for some small molecules. Compared with other fluorescent reagents that label amino groups such as FITC, TRITC, etc., Cyanine5 NHS ester has a higher reaction selectivity.
Cyanine5 NHS ester is a near-infrared dye commonly used for in vivo imaging of small animals, in addition to labeling peptides, proteins, and oligonucleotide amino groups. Because it avoids the visible light region that many substances absorb, the absorbed near-infrared light penetrates deeper into biological tissues, and the excited fluorescence is less affected by the background of biological tissues, so it can generate signals in deep tissues. Especially in the range of 700-900 nm, the absorption of water and hemoglobin is minimal, and near-infrared light can penetrate up to 15 cm deep into tissues. At the same time, these dyes also have biological safety that UV dyes and isotope labeling cannot possess. Cyanine 5 (cy 5) is the most commonly used near-infrared fluorescent dye.
Cyanine 5 NHS ester requires a cosolvent, but due to its weaker hydrophobicity than Cyanine 7, it is more widely used. Li Ji Biotech provides Cyanine 5 NHS ester in the form of triethylamine salts, which is more easily soluble than other companies' potassium salt forms of Cyanine 5 NHS ester. Under the same optical properties and reactivity, it has better solubility. Conjugated NHS ester (succinimide ester) is often used to label amino groups of biomolecules. Maleimide is often used to label thiol groups.
Cyanine 5.5 NHS ester has broad-spectrum properties similar to Alexa Fluor 680 NHS ester and DyLight 680 NHS ester, and can be used as a substitute.
technical information
1.Ex(nm)：649
2.Em(nm)：665
3.molecular weight：855.07
4.solvent：DMSO
Product components
Product Name | Specifications |
Cyanine5 NHS ester (be better thancycy5 NHS ester) | 1 mg |
Transportation and storage
Blue ice transportation- Store at 20 ℃ away from light, with a shelf life of 12 months.
This product is only for scientific experimental research and should not be used in clinical diagnosis, treatment, or other fields.
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