product description
Rui's dye is a composite dye composed of the acidic dye eosin and the alkaline dye methylene blue. Its staining characteristics are: hemoglobin, eosinophilic granules are alkaline proteins, which bind with the acidic dye eosin and stain pink. The nuclear protein is an acidic protein that binds to alkaline dyes such as methylene blue or azurol and dyes purple blue. Neutral particles are in an isoelectric state and can bind to both eosin and methylene blue, dyeing light purple.
Jimsa staining solution is composed of sky blue and eosin, and its staining principle is similar to the Wright staining method, but its staining effect on cytoplasm and neutral granules is weaker. To combine the advantages of both, it is recommended to use the Wright Giemsa composite staining method, which can more comprehensively display cell structure and composition.
ordering information
product name | Item number | specifications |
Ruishi Jimsa staining solution | AS11L212 | 100mL |
Transportation and storage
Transport at room temperature. Store at room temperature and avoid light, with a shelf life of 12 months.
Usage
1. Smear and fix: Spread the cells evenly on a clean glass slide, and fix them after drying.
Note: The choice of fixative depends on the specific situation, and most cells can be fixed with methanol. Methanol fixation: After drying the smear, immerse it in methanol for 15-30 minutes for fixation.
2. Staining:
(1) After fixation, ventilate and dry at room temperature. Place the slide in the dye vat and prepare for dyeing.
(2) Pour the Wright Giemsa dye solution into the dye vat to cover the cells on the slide. The specific staining time depends on the cells, usually 3-30 minutes, and it is best to observe under a microscope during staining.
(3) After staining, wash with water. Rinse the slide with PBS solution first, and then rinse with a small stream of water to prevent a large number of cells from falling off the slide and affecting subsequent observation. After the film processing is completed, place the slide in a cool place to air dry.
3. Sealing: Neutral gum sealing, made into a permanent smear.
4. Microscopic observation: Place the dried slide under a microscope for observation.
Staining result: The nucleus is stained blue, while the cytoplasm is stained red.
matters needing attention
1. This product is only for scientific experimental research and should not be used in clinical diagnosis, treatment, or other fields.
2. For your safety and health, please wear lab clothes and disposable gloves when operating.
3. The staining time is related to room temperature and the number of cells. If the room temperature is low and there are many cells, the staining time will be longer; On the contrary, it can reduce staining time. If necessary, increase the amount of dye or extend the time. Before rinsing, it is necessary to observe under a low-power microscope whether the nucleated cells are clearly stained and whether the nuclear cytoplasm is distinct. We should explore the appropriate time in specific practice, especially when changing the dye solution. Each batch of staining solution needs to be tested for dyeing in order to determine the dyeing time.
4. When rinsing, the dye solution should not be poured out first. It should be rinsed with running water, otherwise the dye will deposit on the carrier.
5. The rinsed slide should be placed upright on a bracket to prevent residual moisture from soaking and causing discoloration.
6. It is recommended to take 1-2 samples for preliminary experiments when using this kit for the first time.
Related product recommendations
Universal tissue cell fixative (4% paraformaldehyde) (item number: AC28L112)
Anti fluorescence quencher (item number: AC28L512)
Jimsa staining solution (item number: AS11L232)
Rui's staining solution (item number: AS11L242)
This product is only for scientific experimental research and should not be used in clinical diagnosis, treatment, or other fields.
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