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EZ Trans Lipo Cell Transfection Reagent (Liposome)

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Product Item Number
Product Specifications
original price
  • AC04L071
    1mL
    1300
Product Description Product FAQ COA Product Literature MSDS

principleProduct Description

EZ Trans Lipo Cell Transfection Reagent (Liposome) (hereinafter referred to as "EZ Trans Lipo") is the latest cell transfection reagent developed by Li Ji Biology. EZ Trans Lipo wraps nucleic acid in nano liposomes and uses a special delivery mechanism to deliver exogenous DNA, RNA, and siRNA into various cells. It can be transfected into adherent cells, suspended cells, and can also be used for siRNA and shRNA gene knockout experiments and gene expression studies.

After comparing and testing nearly a hundred types of cells, EZ Trans Lipo showed the same or better transfection efficiency and cytotoxicity performance as imported products in the vast majority of the tested cells; It has the advantages of high transfection efficiency, low cytotoxicity, wide applicability to cells, and high cost-effectiveness.

More cases can be viewed and queried on the official website under "Resource Support" and "Case Appreciation".

Product components

Product Name

Specifications

EZ Trans Lipo Cell Transfection Reagent (Liposome)

1 mL


Transportation and storage

Blue ice transportation. Stored at 4 ℃, with a shelf life of 12 months.

Attachment: Comparison of Several Cell Transfection Methods

Transfection method

principle

Main applications

characteristic

Cationic polymer
EZ Trans

Positively charged polymers form positively charged complexes with negatively charged phosphate groups in nucleic acids, which then interact with negatively charged proteoglycans on the cell surface and enter the cell through endocytosis.

Stable transfection

Instantaneous transfection

All cells

In addition to the high transfection efficiency, simple operation, wide applicability, and good repeatability of cationic liposomes, it also has the characteristics of high transfection efficiency and low cytotoxicity in vivo, making it a new generation of transfection reagents.

Cationic liposome method

EZ Trans lipo pro

Positively charged liposomes form complexes with negatively charged phosphate groups in nucleic acids, and then the remaining nuclei on the liposomes bind to the negatively charged nuclei of sialic acid residues on the cell membrane; In addition, cells can also engulf DNA encapsulated nanoliposome particles into cells through endocytosis.

Stable transfection

Instantaneous transfection

All cells

The usage method is simple, it can carry large fragments of DNA, and is applicable to various types of naked DNA, RNA, or siRNA. It can be transfected into various types of cells without immunogenicity. It has high efficiency in in vitro gene transfection.

DEAE glucan method

The complex formed by the interaction between positively charged DEAE glucan and negatively charged phosphate backbone of nucleic acid is internalized by cells

Instantaneous transfection

Relatively simple and reproducible compared to calcium phosphate, but it has certain toxic side effects on cells. Serum needs to be removed during transfection and is generally only used for BSC-1, CV-1, COS cell lines

Calcium phosphate method

Calcium phosphate DNA complex adsorbed onto cell membrane and internalized by cell

Stable transfection

Transfection with dye

Not suitable for primary cells (requiring high DNA concentration), easy to operate but with poor repeatability. Some cells are not suitable. CSCL gradient centrifugation is recommended for cells, as transfection requires a high copy number

Retrovirus (RNA)

It enters the host cell through the interaction between the virus membrane glycoprotein and the receptor on the host cell surface, and then reverses into an enzyme to initiate the synthesis of DNA, which is randomly integrated into the host genome

Stable transfection

Specific host cells

Can be used for difficult to transfect cells, primary cells, in vivo cells, etc., but the gene carrying capacity should not be too large

adenovirus

(Double stranded DNA)

First, it binds to the receptors on the cell surface, and then is engulfed by the cell under the mediation of α v integrin

Instantaneous transfection

Specific host cells

Can be used for difficult to transfect cells, safety factors need to be considered

Biolistic Particle Transfer Method

(Gene gun particle bombardment method)

The DNA is precipitated using microscopic heavy metal particles, and then the coated particles are projected into the cell using a ballistic device. The DNA is gradually released inside the cell and expressed

Transient transfection

Stable transfection

Can be used for: human epidermal cells, fibroblasts, lymphocyte lines, and primary cells

Microinjection method

Directly inject DNA into the target cell nucleus through microscopic manipulation

Stable transfection

Instantaneous transfection

The number of transfected cells is limited, and they are mostly used for engineering or transgenic animal embryonic cells

Electroporation method

High pulse voltage disrupts the cell membrane potential, and DNA is introduced through small pores formed on the membrane

Stable transfection

Instantaneous transfection

All cells

Widely applicable, in addition to plasmids, it can also be transfected into large genomes (>65kb), but the cell lethality is high, and the amount of DNA and cells used is large. Therefore, the electroporation experimental conditions need to be optimized according to different cell types, with copy numbers ranging from 1 to 20


This product is only for scientific experimental research and should not be used in clinical diagnosis, treatment, or other fields.

Fill in the product batch number for querying

Fill in the product batch number for querying

Contact Information

Landline Phone Number:021-50778506 
Service Manager:18616108315

Address:4th Floor, Building 27, Lane 908, Ziping Road, Pudong New Area International Medical Park, Shanghai

E-mail:lsj0027@obiosh.com

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