Product Description
Tissue dissociation fluid is a mixture of various biological enzymes that can gently, quickly, and efficiently hydrolyze connective tissue enzymes in tissues and release them into cells, forming a cell suspension. Tissue dissociation solution is suitable for cell separation of various tissues such as spleen, liver, kidney, pancreas, gastric mucosal tissue, tumor tissue, etc. Cells obtained from dissociated tissues have high activity and can retain surface antigens. Cells can be used for cell culture, cell sorting, drug screening, single-cell sequencing, etc.
ordering information
Product Name | Item number | Specifications |
Organizational dissociation fluid | AC05L322 | 10mL |
Transportation and storage
Blue ice transportation- Stored at 20 ℃, with a shelf life of 6 months.
Product parameters
Appearance： | Light yellow clear liquid |
sterile： | Aseptic growth |
mycoplasma： | negative |
Experimental preparation
Reagent: Cell culture medium, red blood cell lysate (item number: AP01L106) (optional)
Consumables: Centrifugal tube, disposable sterile filter (70 µ m)
Equipment: ophthalmic scissors, ophthalmic forceps
Instruments: Constant temperature shaker, centrifuge
usage method
1. Take about 100mg of fresh tissue and add it to a sterile centrifuge tube. Use an ophthalmic scissors to thoroughly shred the tissue to 1-2mm3 and add 1mL of tissue digestion solution.
2. Place the centrifuge tube on a constant temperature shaker, shake and incubate at 37 ℃ and 180rpm for 10-30 minutes to digest the tissue. Depending on the different tissues, the digestion time can be appropriately shortened or extended.
3. After incubation, filter the tissue homogenate in the centrifuge tube through a 70 µ m sterile filter, then rinse the filter with cell culture medium and collect the filtrate using a centrifuge tube.
4. Centrifuge the cell suspension at 1200rpm for 5 minutes and discard the supernatant.
5. Add an appropriate amount of culture medium, centrifuge at 1200rpm for 5 minutes, and discard the supernatant.
6. Resuspend cells in cell culture medium and take a small amount of cells to observe digestion under a microscope.
7. Use cells for subsequent experiments as needed.
8. (Optional) Red blood cell lysis buffer can be used to remove red blood cells or dead cells during tissue dissociation.
matters needing attention
1. This product is only for scientific experimental research and should not be used in clinical diagnosis, treatment, or other fields.
2. After receiving the tissue dissociation solution, it should be divided according to the actual usage amount, stored at -20 ℃, and taken as needed to avoid repeated freezing and thawing.
3. The organization dissociation solution should not be stored at 4 ℃ for more than 48 hours, as prolonged storage at 4 ℃ will reduce the dissociation effect of the product.
4. If the cells obtained from tissue dissociation are used for cell culture, all operations should be carried out under sterile conditions.
This product is only for scientific experimental research and should not be used in clinical diagnosis, treatment, or other fields.
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