Product Description
UMR-106 (Chinese name: rat osteosarcoma cells) is derived from rat osteosarcoma cells, with normal cell morphology resembling epithelial cells and adherent growth.
ordering information
Product Name | Item number | Specifications |
UMR-106 | AX41L205 | T25/1 mL |
type of shipping
Transport at room temperature/dry ice.
usage method
Cultivation methods
culture medium | DMEM （GIBCO: C11965500BT）90%， Fetal Bovine Serum (high-quality fetal bovine serum) or Foetal Bovine Serum (item number: AC03L055) 20% |
Cultivation conditions | 37℃ 5% CO2 |
Digestive conditions | 0.25% (w/v) Trypsin-0.53 mM EDTA solution, digest for 1-2 minutes |
Passage ratio | 1：2-1：4 |
Fluid change time | Change the fluid every 2-3 days |
Freezing method
Frozen storage solution | 55% medium, 40% FBS, 5% (v/v) DMSO or Heyuan Liji rapid cell cryopreservation solution (serum-free, protein free) (item number: AC05L033) |
Freezing density | 1-3 ×106 /Tube, stored in liquid nitrogen |
For specific operation methods, please refer to the cell user manual
matters needing attention
This product is only for scientific experimental research and should not be used in clinical diagnosis, treatment, or other fields.
After receiving the cells, please carefully read the cell instructions, operate according to the regulations, and record the cell status (take photos). Our company provides customers with a one week after-sales guarantee, and in certain circumstances, cells can be resent for free. If you encounter technical problems, please contact 021-50778506 for technical support and provide pictures life-ilab@obiosh.com .
Customization is available as needed. If you have further modifications (such as gene silencing, gene knockout/knock in, gene overexpression, etc.) to the purchased cell line, please contact Heyuan Biotechnology Service at 021-585887-805615800353038.
参考文献
Banerjee C, et al. An AML-1 consensus sequence binds an osteoblast-specific complex and transcriptionally activates the osteoclacin gene. Proc. Natl. Acad. Sci. USA 93: 4968-4973, 1996. PubMed: 8643513
.Endocrinology of calcium metabolism. Amsterdam: Elsevier/North Holland; 1977.
Atkins D, et al. Rat osteogenic sarcoma cells: modulation of hormone stimulated cyclic AMP production by prostaglandin antagonists and biosynthesis inhibitors. Clin. Exp. Pharmacol. Physiol. 7: 31-44, 1980. PubMed: 6103768
Manolagas SC, Deftos LJ. Cytoreceptor assay for 1,25-dihydroxyvitamin D3: a novel radiometric method based on binding of the hormone to intracellular receptors in vitro. Lancet 2: 401-402, 1980. PubMed: 6105521
Partridge NC, et al. Receptors for 1,25(OH)2-vitamin D3 enriched in cloned osteoblast-like rat osteogenic sarcoma cells. FEBS Lett. 115: 139-142, 1980. PubMed: 6248375
Related product recommendations
Foetal Bovine Serum (SKU: AC03L055)
RPMI-1640 medium (containing L-glutamine) (item number: AC01L064)
This product is only for scientific experimental research and should not be used in clinical diagnosis, treatment, or other fields.
Fill in the product batch number for querying
Fill in the product batch number for querying
Back
