Cy3 TUNEL Assay Apoptosis Detection Kit-apoptotic process-He Yuan Li Ji

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Cy3 TUNEL Assay Apoptosis Detection Kit

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Product Item Number

Product Specifications

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AC12L084

20T

1180
AC12L085

50T

2280

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Product Description

A significant characteristic of cell apoptosis is the degradation of chromosomal DNA, which is a common phenomenon. This degradation is very specific and regular, and the resulting DNA fragments of different lengths are about 180 bp-200 bp integer multiples, which is shown by the unique ladder pattern in the agarose gel electrophoresis. This kit adopts the TUNEL method, and uses Terminal Deoxyribonucleotide Transfer (TdT) to catalyze the incorporation of Cy3 dUTP into the 3 ´ - OH end of apoptotic cell broken DNA. Cy3 dUTP labeled DNA can be directly observed under a fluorescence microscope or quantified using a flow cytometer. The TUNEL method can selectively detect apoptotic cells, rather than necrotic cells or cells with DNA strand breaks caused by irradiation and drug therapy. In the TUNEL experiment, TdT enzyme catalyzes the incorporation of dUTP into the 3 ′ - OH end of a broken DNA strand. Antigen labeled dUTP (such as digoxin dUTP, biotin dUTP) is a faster and more direct detection method because it can be directly detected in situ.

Product components

Components	20 T	50 T
A.Cy3 TUNEL Reaction Buffer	1 mL	2 × 1.25 mL
B.TdT Enzyme	20 μL	50 μL
C.Proteinase K (2 mg/mL)	40 μL	100 μL
D.DNase I (2 U/μL)	5 μL	13 μL
E.10 × DNase I Buffer	100 μL	260 μL

Transportation and storage

蓝冰运输。-20℃避光保存，有效期24个月。【注】：避免反复冻融。

This product is only for scientific experimental research and should not be used in clinical diagnosis, treatment, or other fields.

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