Product Description
NCI-H446 (Chinese name: Human Small Cell Lung Cancer Cell), derived from small cell lung cancer; The normal cell morphology is epithelial cells that grow adherent to the wall.
ordering information
Product Name | Item number | Specifications |
NCI-H446 | AX03L931 | T25/1mL |
type of shipping
Transport at room temperature/dry ice.
usage method
Cultivation methods
culture medium | RPMI-1640 （Gibco：C11875）90%, Fetal Bovine Serum (high-quality fetal bovine serum) or Foetal Bovine Serum (item number: AC03L055) 10% |
Cultivation conditions | 37℃ 5% CO2 |
Digestive conditions | 0.25% Trypsin- EDTA(GIBCO: 25200-056)，Digestion1-3min |
Passage ratio | 1：3-1：4 |
Fluid change time | Change the fluid every 2-3 days |
Freezing method
Frozen storage solution | 85% medium, 10% FBS, 5% (v/v) DMSO or Heyuan Liji rapid cell cryopreservation solution (serum-free, protein free) (item number: AC05L033) |
Freezing density | 1-3 ×106 /Tube, stored in liquid nitrogen |
For specific operation methods, please refer to the cell user manual
matters needing attention
This product is only for scientific experimental research and should not be used in clinical diagnosis, treatment, or other fields.
After receiving the cells, please carefully read the cell instructions, operate according to the regulations, and record the cell status (take photos). Our company provides customers with a one week after-sales guarantee, and in certain circumstances, cells can be resent for free. If you encounter technical problems, please contact 021-50778506 for technical support and provide pictures life-ilab@obiosh.com .
Customization is available as needed. If you have further modifications (such as gene silencing, gene knockout/knock in, gene overexpression, etc.) to the purchased cell line, please contact Heyuan Biotechnology Service at 021-585887-805615800353038.
reference
Little CD, et al. Amplification and expression of the c-myc oncogene in human lung cancer cell lines. Nature 306: 194-196, 1983. PubMed: 6646201
Carney DN, et al. Establishment and identification of small cell lung cancer cell lines having classic and variant features. Cancer Res. 45: 2913-2923, 1985. PubMed: 2985257
Gazdar AF, et al. Characterization of variant subclasses of cell lines derived from small cell lung cancer having distinctive biochemical, morphological, and growth properties. Cancer Res. 45: 2924-2930, 1985. PubMed: 2985258
Verbeeck MA, et al. Expression of the vasopressin and gastrin-releasing peptide genes in small cell lung carcinoma cell lines. Pathobiology 60: 136-142, 1992. PubMed: 1320893
Related product recommendations
Foetal Bovine Serum (SKU: AC03L055)
RPMI-1640 medium (containing L-glutamine) (item number: AC01L064)
This product is only for scientific experimental research and should not be used in clinical diagnosis, treatment, or other fields.
Fill in the product batch number for querying
Fill in the product batch number for querying
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