Product Description
A172 (Chinese name: human glioblastoma cells), derived from the brain; Glioblastoma, adherent growth.
ordering information
Product Name | Item number | Specifications |
A172 | AX04L301 | T25/1mL |
type of shipping
Transport at room temperature/dry ice.
usage method
Cultivation methods
culture medium | DMEM （GIBCO: 12800-017）90%, Fetal Bovine Serum (high-quality fetal bovine serum) or Foetal Bovine Serum (item number: AC03L055) 10% |
Cultivation conditions | 37℃ 5% CO2 |
Digestive conditions | 0.25% (w/v) Trypsin-0.53 mM EDTA solution, digest for 1-3 minutes |
Passage ratio | 1：2-1：3 |
Fluid change time | Change the fluid every 2-3 days |
Freezing method
Frozen storage solution | 85% medium, 10% FBS, 5% (v/v) DMSO or Heyuan Liji rapid cell cryopreservation solution (serum-free, protein free) (item number: AC05L033) |
Freezing density | 1-3 ×106 /Tube, stored in liquid nitrogen |
For specific operation methods, please refer to the cell user manual
matters needing attention
This product is only for scientific experimental research and should not be used in clinical diagnosis, treatment, or other fields.
After receiving the cells, please carefully read the cell instructions, operate according to the regulations, and record the cell status (take photos). Our company provides customers with a one week after-sales guarantee, and in certain circumstances, cells can be resent for free. If you encounter technical problems, please contact 021-50778506 for technical support and provide pictures life-ilab@obiosh.com .
Customization is available as needed. If you have further modifications (such as gene silencing, gene knockout/knock in, gene overexpression, etc.) to the purchased cell line, please contact Heyuan Biotechnology Service at 021-585887-805615800353038.
reference
Olopade OI, et al. Molecular analysis of deletions of the short arm of chromosome 9 in human gliomas. Cancer Res. 52: 2523-2529, 1992. PubMed: 1568221
Giard DJ, et al. In vitro cultivation of human tumors: establishment of cell lines derived from a series of solid tumors. J. Natl. Cancer Inst. 51: 1417-1423, 1973. PubMed: 4357758
Debinski W, et al. Receptor for interleukin (IL) 13 does not interact with IL4 but receptor for IL4 interacts with IL13 on human glioma cells. J. Biol. Chem. 271: 22428-22433, 1996. PubMed: 8798406
This product is only for scientific experimental research and should not be used in clinical diagnosis, treatment, or other fields.
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