Protease K-DNA extraction-He Yuan Li Ji

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Protease K

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Product Item Number

Product Specifications

original price

AP43L035

100mg

185
AP43L036

1g

1350

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Product Description Product FAQ COA Product Literature MSDS

Product Description

Protease K, with a relative molecular weight of approximately 29.3 kDa, is a serine protease that can cleave the carboxyl terminal peptide bonds of both aliphatic and aromatic amino acids. It has a wide range of cleavage activities. It has activity in a wide pH range (pH 4-12.5). The general working concentration is 50-100 µ g/ml, but it can enhance activity in denaturing agents such as 1% SDS. Therefore, the specific working concentration is often determined based on factors such as SDS, urea, pH, temperature, etc. in the buffer solution used during use. Common concentrations of EDTA Triton X-100、Tween 20、Sarkosyl、 Guanidine hydrochloride has little effect on the activity of protease K.

Commonly used for experiments such as genome DNA extraction and preparation of chromosomal DNA by pulse electrophoresis.

Product components

Product Name

Specifications

Protease K

100 mg

Protease K

1 g

Transportation and storage

Blue ice transportation- Stored at 20 ℃, with a shelf life of 24 months. [Note]: Avoid repeated freezing and thawing.

This product is only for scientific experimental research and should not be used in clinical diagnosis, treatment, or other fields.

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