Product Description
The BCA (Bicinchonic acid) method is currently a widely used protein concentration determination method. Based on the biuret reaction, which involves the protein reducing Cu2+to Cu+in an alkaline environment, Cu+forms a purple colored complex with BCA reagent, with a high absorbance at 562 nm. The amount of the reaction product is proportional to the protein concentration. Measure its absorbance at 562 nm and compare it with the standard curve to calculate the concentration of the protein to be tested. This method is fast, sensitive, stable, reliable, and has a small coefficient of variation for different types of proteins. The reagent kit provides protein standards with stable concentration for making standard curves.
The BCA protein quantification kit can be used for in vitro or microplate detection. The test tube method requires a large amount of protein sample (100 μ L) and working solution (2 mL). The ratio of protein sample to BCA working solution is 1:20 (v/v), and the protein determination range is 20-2000 μ g/mL. The enhanced method can detect 5 μ g/mL; The microplate method is simple and convenient to operate, requiring only a small amount (10-25 μ L) of protein sample and working solution (200 μ L). The ratio of protein sample to working solution is 1:20 (v/v) or 1:8 (v/v), and the protein determination range is 20-2000 μ g/mL. The enhanced method can detect 5 μ g/mL.
The BCA protein concentration detection kit provided by our company includes 50 colorimetric dishes and 500 enzyme-linked immunosorbent assay (ELISA) tests.
产品组分
Item number | Components | Specifications |
AP12L025 | BCA reagent A | 100mL |
BCA reagent B | 5mL |
BSA Standard product2 mg/mL | 2*1mL |
Transportation and storage
Transport at room temperature. Stored at room temperature, with a shelf life of 12 months.
BCA compatibility table
Appendix Compatibility of BCA protein concentration determination
name | Tolerant concentration | name | Tolerant concentration |
Sodium bicarbonate | 100 mM | BugBuster protein Extraction Reagent | nointerference(undiluted) |
Sodium phosphate | 25 mM | Calcium chloride (in TBS, pH8.0) | 10 mM |
2-Mercaptoethanol | 0.01% | CelLytic B Reagent | nointerference(undiluted) |
Glycercol (pure) | 10% | Cesium bicarbonate | 100 mM |
Glycine-HCl, pH2.8 | 100 mM | CHAPS | 5% |
HEPES | 100 mM | Cobalt chloride (in TBS, pH8.0) | 0.8 mM |
Hydrochloric acid | 100 mM | CytoBuster Protein Extraction Reagent | nointerference(undiluted) |
Leupeptin | 10 mg/L | Deoxycholic acid | 5% |
Nickel chloride (in TBS, pH8.0) | 10 mM | Dithioerythritol (DTE) | 1mM |
Nonidet P-40 (NP-40) | 5% (w/v) | Dithiothreitol (DTT) | 1mM |
Octyl β-glucoside | 5% (w/v) | DMF | 10% |
Potassium thiocyanate | 3.0 M | DMSO | 10% |
SDS | 5% | EDTA | 10 mM |
Sodium acetate, pH4.8 | 200 mM | EPPS, pH8.0 | 100 mM |
Sodium azide | 0.20% | Ethanol | 10% |
Sodium hydroxide | 100 mM | Ferric chloride (in TBS, pH8.0) | 10 mM |
Sucrose | 40% | Glucose | 10 mM |
Triton X- 100 | 5% | Glycerol | 10% |
Triton X- 114, X-305, X-405 | 1% | Guanidine-HCl | 4 M |
Tween-20, Tween-60, Tween-80 | 5% | Imidazole, pH7.0 | 50 mM |
Zwittergent | 1% | MES, PH6. 1 | 100 mM |
ACES, pH7.8 | 25 mM | Methanol | 10% |
Acetone | 10% | MOPS, pH7.2 | 100 mM |
Acetonitrile | 10% | N-Acetyglucosamine (10mM) in PBS, pH7.2 | 10 mM |
Ammonium sulfate | 1.5 mM | Octyl β – thioglucpyranoside | 5% |
Aprotinin | 10 mg/L | PIPES, pH6.8 | 100 mM |
Bicine, pH8.4 | 20 mM | PMSF | 1mM |
Bis-Tris, pH6.5 | 33 mM | PopCulture Reagent | nointerference(undiluted) |
Borate, pH8.5 | 50 mM | Reportasol Extraction Buffer | nointerference(undiluted) |
Brij-35 | 5% | Sodium chloride | 1 M |
Brij-52 | 1% | Sodium citrate, pH4.8 or pH6.4 | 200 mM |
Brij-56, Brij-58 | 1% | Sodium ortho-vanadate in PBS, pH7.2 | 1mM |
Triethanolamine, pH7.8 | 25 mM | Span 20 | 1% |
Tris | 250 mM | TBS (150 mM NaCl, 100 mM Tris-HCl,pH8.0) | nointerference(undiluted) |
Tris (hydroxypropyl) phosphine (THP) | 1mM | Thimerosal | 0.01% |
Urea | 3 M | TLCK | 0. 1mg/L |
Zinc chloride (in TBS, pH8.0) | 10 mM | TPCK | 0. 1mg/L |
This product is only for scientific experimental research and should not be used in clinical diagnosis, treatment, or other fields.
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