Product Description
IFluor dyes are a series of excellent fluorescent labeling dyes that can cover the entire visible spectrum. All iFluor dyes have excellent water solubility. Their hydrophilicity minimizes the use of organic solvents. IFluor dyes also have better labeling performance than classical fluorescent labeling dyes such as FITC, TRITC, Texas Red, Cy3, Cy5, and Cy7. Some iFluor dyes are significantly superior to Alexa Fluor labeled dyes on certain antibodies. They are extremely inexpensive fluorescent dyes used to label proteins and nucleic acids without performance (replacing Alexa Fluor dyes). The development of each iFluor dye is matched with the spectral characteristics of specific Alexa Fluor or other marker dyes (such as DyLight dye).
Succinimide based (NHS) esters have been proven to be excellent reagents for amine modification, as the formed amide bonds are essentially identical and stable to natural peptide bonds. These reagents are usually stable and exhibit good reactivity and selectivity with aliphatic amines. When succinimide ester compounds are used for conjugation reactions, there are few factors to consider: 1. Solvent: In most cases, the reactive dye should be dissolved in anhydrous dimethylformamide (DMF) or dimethyl sulfoxide (DMSO). 2: Reaction pH: The labeling reaction between amine and succinimide ester strongly depends on pH. Amine reactive reagents react with non protonated aliphatic amine groups, including the terminal amine of proteins and the beta amino group of lysine. Therefore, the acylation reaction is usually carried out at pH 7.5 or above. Protein modification through succinimide esters can typically be carried out at pH 8.5-9.5. 3: Reaction buffer: When using amine reaction reagents, it is necessary to avoid using buffers containing free amines (such as Tris and glycine) and thiol compounds. Ammonium salts widely used for protein precipitation, such as ammonium sulfate and ammonium acetate, must also be removed before dye conjugation (e.g. by dialysis). 4: Reaction temperature: Most conjugations are carried out at room temperature. However, specific labeling reactions may require an increase or decrease in temperature.
technical information
1.Ex(nm)：786
2.Em(nm)：811
3.molecular weight：1768.30
4.solvent：DMSO
Product components
Product Name | Specifications |
IFluor 790 succinimide ester (Cy7 upgraded product) | 1 mg |
Transportation and storage
Blue ice transportation- Store at 20 ℃ away from light, with a shelf life of 12 months.
This product is only for scientific experimental research and should not be used in clinical diagnosis, treatment, or other fields.
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