Product Description
143B (Chinese name: human osteosarcoma cell) originates from human bone or cartilage, and its normal cell morphology is a mixed type, consisting of fibroblast like and epithelioid cells, growing adherent to the wall.
ordering information
Product Name | Item number | Specifications |
143B | AX01L111 | T25/1 mL |
type of shipping
Transportation at room temperature/dry ice。
usage method
Cultivation methods
culture medium | Minimum essential medium (Eagle) in Earle’s BSS with 0.015 mg/ml 5-bromo-2′-deoxyuridine 90%, Fetal Bovine Serum (High quality fetal bovine serum) or Foetal Bovine Serum (product code: AC03L055) 10% |
Cultivation conditions | 37℃ 5% CO2 |
Digestive conditions | 0.25% (w/v) Trypsin-0.53 mM EDTA solution, digest for 1-3 minutes |
Passage ratio | 1：3-1：5 |
Fluid change time | Change the fluid every 2-3 days |
Freezing method
Frozen storage solution | 90%FBS, 10% (v/v) DMSO Or the rapid cell cryopreservation solution of Yuanliji (serum-free, protein free) (item number: AC05L033) |
Freezing density | 1-3 ×106 /Tube, stored in liquid nitrogen |
For specific operation methods, please refer to the cell user manual
matters needing attention
This product is only for scientific experimental research and should not be used in clinical diagnosis, treatment, or other fields.
After receiving the cells, please carefully read the cell instructions, operate according to the regulations, and record the cell status (take photos). Our company provides customers with a one week after-sales guarantee, and in certain circumstances, cells can be resent for free. If you encounter technical problems, please contact 021-50778506 for technical support and provide pictures life-ilab@obiosh.com .
Customization is available as needed. If you have further modifications (such as gene silencing, gene knockout/knock in, gene overexpression, etc.) to the purchased cell line, please contact Heyuan Biotechnology Service at 021-585887-805615800353038.
Related product recommendations
Foetal Bovine Serum (SKU: AC03L055)
RPMI-1640 medium (containing L-glutamine) (item number: AC01L064)
This product is only for scientific experimental research and should not be used in clinical diagnosis, treatment, or other fields.
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