Product Description
Serum contains proteins, amino acids, glucose, hormones, etc., among which proteins are mainly albumin and globulin. There are various types of amino acids, which are the basic components of protein synthesis in cells. Some of these amino acids cannot be synthesized by animal cells themselves (known as essential amino acids). The hormones contained in serum include insulin, growth hormone, and various growth factors (such as epidermal growth factor, fibroblast growth factor, insulin-like growth factor, etc.). Serum also contains various unknown factors that promote cell growth, adhesion, and other active substances, which can promote cell growth, proliferation, and adhesion.
Each batch of raw blood material from Li Ji Biology's fetal bovine serum has undergone strict screening and has been filtered through three consecutive layers of 0.1 μ m filter membranes. The filter material meets the requirements of the US FDA regulations. The entire production process complies with international fetal bovine serum production standards and undergoes strict quality control and analysis and testing of physical, chemical, and microbiological indicators. Among them, multiple indicators such as pH value, osmotic pressure, hemoglobin concentration, endotoxin level, and total protein have been tested and qualified. There is no bacterial, fungal, mycoplasma, or viral contamination, and the quality between batches is stable. It is suitable for various cell cultures and can also be used for the cultivation of primary cells and some stem cells.
ordering information
product name | Item number | specifications |
Defined FBS (Foetal Bovine Serum) | AC03L155 | 500 mL |
Defined FBS (Foetal Bovine Serum) | AC03L156 | 10*50 mL |
Transportation and storage
Dry ice transportation. Short term storage at 4 ℃- Stored at 15 ℃~-40 ℃, with a shelf life of 60 months.
matters needing attention
1. This product is only for scientific experimental research and should not be used in clinical diagnosis, treatment, or other fields.
2. Thawing and Storage of Serum: After taking out the serum from a -20 ℃ or lower temperature freezer, it is recommended to thaw it in a 4 ℃ freezer. Gently shake it evenly every once in a while, and do not directly thaw it at a higher temperature to avoid protein aggregation and precipitation caused by significant temperature changes, which can lead to a decrease in serum quality. If a bottle cannot be used up in the short term, it is recommended to pack it aseptically and then put it back in the freezer to avoid repeated freeze-thaw cycles. After long-term storage at 4 ℃, the denatured lipoprotein and fibrin in the serum form flocculent substances that deteriorate.
3. Serum inactivation: Heating can inactivate the complement system in serum. In rare cases (when culturing ES cells and smooth muscle cells), inactivation is recommended. Serum inactivation is not recommended when culturing other cells. Serum inactivation is very prone to precipitation. If inactivation is necessary, please operate according to the principle of 56 ℃, 30 min, and slight shaking. High inactivation temperature, long time, and uneven shaking are all prone to precipitation.
4. Serum free culture medium: Serum free culture medium has advantages in repeatability of results and in vitro cell differentiation, but serum is still the most basic additive in the culture medium, especially in primary culture or when cell growth is poor. Serum containing culture medium is often used for cultivation first, and then replaced with serum-free culture medium when cell growth is vigorous.
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This product is only for scientific experimental research and should not be used in clinical diagnosis, treatment, or other fields.
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