product description
The one-stop mouse tail genotype rapid identification kit is suitable for quickly extracting genomic DNA from mouse tail, ear, toe or other tissues, and performing PCR amplification to achieve rapid, convenient and accurate mouse genotype identification and genome related analysis.
This kit can quickly digest and extract genomic DNA from mouse tail tissue samples within 10-15 minutes, effectively simplifying the process of obtaining mouse tail genomic DNA. This kit also provides a pre prepared 2X PCR Master Mix (Green), which contains Taq DNA Polymerase, PCR Buffer, dNTP, and loading buffer. PCR amplification can be performed by adding appropriate primers, templates, and water. After PCR is completed, the product can be directly analyzed by electrophoresis, which is convenient and fast.
Recommended organizational usage
3~5mm2 mouse tail tip (2~3mm)
5-10mm2 mouse ear (3-5mm)
1 mouse toe
Order Information
product name | Item number
| specifications |
One stop Mouse Tail Gene Rapid Identification Kit | AN11L217 | 200 T |
One stop Mouse Tail Gene Rapid Identification Kit | AN11L218 | 500 T |
Product components
components | AN11L217 | AN11L218 | storage condition |
A. DNA extraction solution | 15 mL | 40 mL | room temperature |
B. Stop solution | 15 mL | 40 mL | room temperature |
C. 2X PCR Master Mix | 3*1 mL | 8*1 mL | -20℃ |
Transportation and storage
Blue ice transportation. The components are stored according to the preservation conditions and have a validity period of 12 months.
usage method
1. Genome extraction steps:
(1) Add 70 μ L of DNA extraction solution to each sample to ensure complete immersion of the tissue in the solution.
(2) Cracking treatment in a metal bath or water bath at 95 ℃ for 10-15 minutes. After lysis, the tissue remains intact in appearance, but a sufficient amount of genomic DNA has been successfully released, which does not affect subsequent PCR experiments.
(3) After cracking, briefly centrifuge to remove the liquid droplets on the inner wall of the tube cover, and then let it stand at room temperature for 2 minutes. Subsequently, 70 μ L of Stop solution was added and gently mixed to terminate the cracking reaction.
(4) Centrifuge at 12000 rpm for 2 minutes, and take the supernatant as the PCR template. The supernatant can be stored at 4 ℃ for one month or at -20 ℃ for one year after digestion.
2. PCR amplification steps:
(1) Place the 2X PCR Master Mix (Green) in an ice bath or ice box, and prepare the primers, templates, and water required for the PCR reaction.
(2) Refer to Table 1, prepare the PCR reaction system on an ice bath.
(3) Gently blow or vortex with a pipette to ensure thorough mixing. Subsequently, centrifuge briefly at room temperature for a few seconds to allow the liquid to concentrate at the bottom of the tube.
(4) Place the prepared PCR reaction system in the PCR machine and initiate the PCR reaction.
(5) Please refer to Table 2 to set the PCR reaction program parameters. After PCR reaction, agarose gel electrophoresis can be carried out directly.
matters needing attention
1. This product is only for scientific experimental research and should not be used in clinical diagnosis, treatment, or other fields.
2. PCR products have few or no bands. Poor primer design is the most common problem and requires rational optimization of primers; Configuring the reaction system at room temperature can easily trigger non-specific amplification, and it is recommended to perform it in an ice bath; Improper annealing temperature may affect the results. It is recommended to use a temperature gradient PCR instrument for optimization or find the optimal temperature through multiple PCR reactions; Insufficient extension time. It is recommended to extend every 1 kb fragment for 1 minute, and for fragments that are difficult to amplify, they can be extended to 1.5-2 minutes.
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This product is only for scientific experimental research and should not be used in clinical diagnosis, treatment, or other fields.
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