product description
Jimsa staining solution is composed of sky blue and eosin, and the staining principle is basically the same as the Wright staining method. The principle is that acidophilic particles are alkaline proteins that bind with the acidic dye eosin to dye it pink; The nuclear protein and lymphocyte cytoplasm are acidic and bind to alkaline dyes such as methylene blue or azurol, staining purple blue; Neutral particles are in an isoelectric state and can bind to both eosin and methylene blue, dyeing light purple.
The dyeing effect is affected by pH. All components of cells contain proteins. As proteins are amphoteric electrolytes, their charge depends on the pH of the solution. In a slightly acidic environment, the positive charge increases, making it easy to bind with eosin and stain reddish; In a slightly alkaline environment, the negative charge increases, making it easy to bind with azurol and stain blue. Therefore, cell staining is highly sensitive to hydrogen ion concentration, and the staining slides must be clean. The rinsing water should be near neutral, otherwise it may cause various abnormal cell staining reactions, making identification difficult.
ordering information
product name | Item number | specifications |
Jimsa staining solution | AS11L232 | 100mL |
Transportation and storage
Transport at room temperature. Store at room temperature and avoid light, with a shelf life of 12 months.
Usage
1. Reagent preparation: Dilute the Giemsa mother liquor 10 times with 0.1M PBS to obtain the Giemsa working solution.
2. Drip film: smear, with uniform density; Alternatively, the cell suspension with adjusted density can be directly dropped onto the glass slide and dried as soon as possible;
3. Fixation: First fix with Jimsa working solution for about 1 minute, then add PBS, and the ratio of the two should not exceed 1:1; Be careful not to dry the film before adding PBS, otherwise there will be a lot of sediment, leading to uneven staining;
4. Staining: The specific staining time depends on the cell, usually 3-30 minutes, and it is best to observe under a microscope during staining;
5. Water washing: When washing the film, pay attention to controlling the water flow. If it is too small, small dye residue may adhere, while if it is too large, it may worsen the peeling process. Stained films can also be modified and compensated for. If the staining is too light, it can be re dyed; If it is too deep, ethanol can be used for instantaneous decolorization.
6. Microscopic examination: Observation under a microscope.
Staining results: The nucleus is stained purple red or purple blue, while the cytoplasm is stained light red.
matters needing attention
1. This product is only for scientific experimental research and should not be used in clinical diagnosis, treatment, or other fields.
2. For your safety and health, please wear lab clothes and disposable gloves when operating.
3. The dyeing working solution should be used and prepared on the spot, as the old dyeing solution has poor dyeing effect. The storage time of working fluid should not exceed 48 hours;
4. Jimsa is extremely sensitive to pH, therefore, the pH of PBS should be accurate, otherwise the staining effect will be poor. When using a staining cylinder for staining, with the extension of staining time, a layer of oxide film (shiny) often forms on the surface of the staining solution, which is easy to adhere to the glass slide and form dirt, and is not easy to remove. Especially when using non freshly prepared reagents, it is easier to form an oxide film. Before dyeing, the oxide layer on the liquid surface should be scraped off with a small piece of filter paper. After staining, the specimen should be immersed in water to rinse the staining solution.
5. It is recommended to take 1-2 samples for preliminary experiments when using this kit for the first time.
Related product recommendations
Universal tissue cell fixative (4% paraformaldehyde) (item number: AC28L112)
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Ruishi Jimsa staining solution (item number: AS11L212)
Rui's staining solution (item number: AS11L242)
This product is only for scientific experimental research and should not be used in clinical diagnosis, treatment, or other fields.
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