Product Description
The Annexin V-FITC/PI cell apoptosis detection kit is a combination of Annexin V and PI, used to detect the occurrence of early apoptosis and distinguish between early apoptotic cells and late apoptotic or necrotic cells.
Annexin V is a member of the intracellular protein membrane associated protein family, which selectively binds to phosphatidylserine (PS) in a calcium dependent manner. PS is normally distributed on the inner side of the cell membrane. PS inversion outside the cell membrane is a prominent feature of different types of cells in the early stages of apoptosis. This important feature can be detected by flow cytometry or fluorescence microscopy using Annexin V labeled with FITC, also known as Annexin V-FITC. FITC exhibits yellow green fluorescence.
PI (Propidium Iodide) is a nuclear staining reagent that can stain DNA, releasing red fluorescence after embedding into DNA. PI cannot penetrate the complete cell membrane, but can penetrate necrotic cells or cells that lose membrane integrity in the late stage of apoptosis.
When Annexin V-FITC is used in combination with PI, PI is excluded from live cells (Annexin V/PI -) and early apoptotic cells (Annexin V+/PI -), while late apoptotic cells and necrotic cells show dual positivity (Annexin V+/PI+) when combined with FITC and PI.
The maximum absorption and excitation wavelengths of FITC are 494nm and 518nm, respectively. The maximum absorption and emission wavelengths of PI-DNA composite are 535nm and 617nm, respectively.
Product advantages
1、 The sensitivity of the Annexin V probe detection reaches the level of nanomolar concentration, and it can detect a very small number of apoptotic cells; Less background interference and clear staining;
2、Stable performance between batches;
3、A variety of apoptosis detection kits are available to meet the diverse needs of different customers.
ordering information
Product Name | Article number | Specifications |
Annexin V-FITC/PICell apoptosis detection kit | AC12L033 | 100 T |
Product components
Composition | Specifications |
A. Annexin V-FITC | 500 μL |
B. Propidium iodide | 1 mL |
C. Binding Buffer(1×) | 100 mL |
Transportation and Preservation
Blue ice transportation. Store at 4 ℃ in dark, with an expiration date of 18 months. [Note]: It cannot be frozen.
This product is limited to scientific experimental research and cannot be used in clinical diagnosis, treatment, or other fields.
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