Product Description
RAW 264.7 (Chinese name: Mouse Monocytic Leukemia Cell) is a mouse monocytic leukemia cell derived from mouse tumors. Its Chinese name is mouse monocytic leukemia cell, and its normal cell morphology is irregular circular with adherent growth.
ordering information
Product Name | Item number | Specifications |
RAW 264.7 | AX31L103 | T25/1mL |
type of shipping
Transport at room temperature/dry ice.
usage method
Cultivation methods
culture medium | DMEM（GIBCO: C11965500BT）90%, Fetal Bovine Serum (high-quality fetal bovine serum) or Foetal Bovine Serum (item number: AC03L055) 10% |
Cultivation conditions | 37℃ 5% CO2 |
Digestive conditions | Discard trypsin in 0.25% (w/v) Trypsin-0.53 mM EDTA solution for about 5 seconds, wait for cells to contract, gently tap and slide in a sandy manner, and terminate digestion |
Passage ratio | 1：3-1：4 |
Fluid change time | Change the fluid every 2-3 days |
Freezing method
Frozen storage solution | 55% medium, 40% FBS, 5% (v/v) DMSO or Heyuan Liji rapid cell cryopreservation solution (serum-free, protein free) (item number: AC05L033) |
Freezing density | 1-3 ×106 /Tube, stored in liquid nitrogen |
For specific operation methods, please refer to the cell user manual
matters needing attention
This product is only for scientific experimental research and should not be used in clinical diagnosis, treatment, or other fields.
After receiving the cells, please carefully read the cell instructions, operate according to the regulations, and record the cell status (take photos). Our company provides customers with a one week after-sales guarantee, and in certain circumstances, cells can be resent for free. If you encounter technical problems, please contact 021-50778506 for technical support and provide pictures life-ilab@obiosh.com .
Customization is available as needed. If you have further modifications (such as gene silencing, gene knockout/knock in, gene overexpression, etc.) to the purchased cell line, please contact Heyuan Biotechnology Service at 021-585887-805615800353038.
reference
Ralph P, Nakoinz I. Antibody-dependent killing of erythrocyte and tumor targets by macrophage-related cell lines: enhancement by PPD and LPS. J. Immunol. 119: 950-954, 1977. PubMed: 894031
Raschke WC, et al. Functional macrophage cell lines transformed by Abelson leukemia virus. Cell 15: 261-267, 1978. PubMed: 212198
Denlinger LC, et al. Regulation of inducible nitric oxide synthase expression by macrophage purinoreceptors and calcium. J. Biol. Chem. 271: 337-342, 1996. PubMed: 8550583
Hambleton J, et al. Activation of c-Jun N-terminal kinase in bacterial lipopolysaccharide-stimulated macrophages. Proc. Natl. Acad. Sci. USA 93: 2774-2778, 1996. PubMed: 8610116
Taylor GA, et al. Identification of anovel GTPase, the inducibly expresed GTPase, that accumulates in response to interferon gamma. J. Biol. Chem. 271: 20399-20405, 1996. PubMed: 8702776
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Foetal Bovine Serum (SKU: AC03L055)
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This product is only for scientific experimental research and should not be used in clinical diagnosis, treatment, or other fields.
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