Product Description
B16-F10 (Chinese name: Mouse Melanoma Cells), derived from mouse skin, has a normal epithelial like cell morphology and grows adherent to the wall.
ordering information
Product Name | Item number | Specifications |
B16-F10 | AX31L533 | T25/1 mL |
type of shipping
Transport at room temperature/dry ice.
usage method
Cultivation methods
culture medium | DMEM 90%, Fetal Bovine Serum (high-quality fetal bovine serum) or Foetal Bovine Serum (item number: AC03L055) 10% |
Cultivation conditions | 37℃ 5% CO2 |
Digestive conditions | 0.25% (w/v) Trypsin-0.53 mM EDTA solution, digest for 1-3 minutes |
Passage ratio | 1：3-1：5 |
Fluid change time | Change the fluid every 2-3 days |
Freezing method
Frozen storage solution | 60% medium, 30% FBS, 10% (v/v) DMSO or Heyuan Liji rapid cell cryopreservation solution (serum-free, protein free) (item number: AC05L033) |
Freezing density | 1-3 ×106 /Tube, stored in liquid nitrogen |
For specific operation methods, please refer to the cell user manual
matters needing attention
This product is only for scientific experimental research and should not be used in clinical diagnosis, treatment, or other fields.
After receiving the cells, please carefully read the cell instructions, operate according to the regulations, and record the cell status (take photos). Our company provides customers with a one week after-sales guarantee, and in certain circumstances, we can resend cells for free. If you encounter technical problems, please contact us in a timely manner021-50778506Transfer technical support and provide pictures tolife-ilab@obiosh.com。
Customization is available upon request. If you have further modifications (such as gene silencing, gene knockout/knock in, gene overexpression, etc.) to the purchased cell line, please contact usHeyuan Biotechnology Service Hotline021-58585887-8056，15800353038。
reference
Fidler IJ. Biological behavior of malignant melanoma cells correlated to their survival in vivo. Cancer Res. 35: 218-224, 1975. PubMed: 1109790
Fidler IJ, et al. Tumoricidal properties of mouse macrophages activated with mediators from rat lymphocytes stimulated with concanavalin A. Cancer Res. 36: 3608-3615, 1976. PubMed: 953987
Fidler IJ, Bucana C. Mechanism of tumor cell resistance to lysis by syngeneic lymphocytes. Cancer Res. 37: 3945-3956, 1977. PubMed: 908034
Fidler IJ, Kripke ML. Metastasis results from preexisting variant cells within a malignant tumor. Science 197: 893-895, 1977. PubMed: 887927
Briles EB, Kornfeld S. Isolation and metastatic properties of detachment variants of B16 melanoma cells. J. Natl. Cancer Inst. 60: 1217-1222, 1978. PubMed: 418183
This product is only for scientific experimental research and should not be used in clinical diagnosis, treatment, or other fields.
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